Always use CAPITAL LETTERS to insert nucleotide sequences (5' to 3'). Never use sequences containing spaces or numbers. The software's input file format is a .txt. Example: 'input_file_name.txt'
The file content for siRNA design: >Gene name 1 GTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGGT AGCTGGGATGTTAGGGCTCAGGGAAGAAAAGTCAGAAGACCAGGACCTCCAGGGCCTCAAGGACAAACCC CTCAAGTTTAAAAAGGTGAAGAAAGATAAGAAAGAAGAGAAAGAGGGCAAGCATGAGCCCTCTCAGCCAT CAGCCTCTCACTCTGCTGAGCCCGCAGAGGCAGGCAAAGCAGAGACATCAGAAGGGTCAGGCTCCGCCCC GGCTGTGCCGGAAGCTTCTGCCTCCCCCAAACAGCGGCGCTCCATCATCCGTGACCGGGGACCCATGTAT GATGACCCTCTCCTGCCTGAAGGCTGGACACGGAAGCTTAAGCAAAGGAAATCTGGCCGCTCTGCTGGGA AGTATGATGTGTATTTGATCAATCCCCAGGGAAAAGCCTTTCGCTCTAAAGTGGAGTTGATTGCGTACTT CGAAAAGGTAGGCGACACATCCCTGGACCCTAATGATTTTGACTTCACGGTAACTGGGAGAGGGAGCCCC TCCCGGCGAGAGCAGAAACTCTCTAAGAAGCCCAAATCTCCCAAAGCTCCAGGAACTGGCAGAGGCCGGG GACGCCCCAAAGGGAGCGGCACCACGAGACCCAAGGCGGCCACGTCAGAGGGTGTGCAGGTGAAAAGGGT CCTGGAGAAAAGTCCTGGGAAGCTCCTTGTCAAGATGCCTTTTCAAACTTCGCCAGGGGGCAAGGCTGAG GGGGGTGGGGCTCTCACATCTCTCCAGGTCATGGTGATCAAACGCCCCGGCAGGAAGCGAAAAGCTGAGG CCGACCCTCAGGCCATTCCCAAGAAACGGGGCCGAAAGCCGGGGAGTGTGGTGGCAGCCGCTGCCGCCGA
is very important to follow the input structure showed above to avoid any errors The output file will be a TXT format and will be created in the software's folder. Example: 'output_file_name_siRNA_Gibbs_SSD_Date_[Hour].txt' for the siRNA designer 'output_file_name_Trivalent_shRNA_Date_[Hour].txt' for the shRNA designer