Always use CAPITAL LETTERS to insert nucleotide sequences (5' to 3'). Never use sequences containing spaces or numbers.
The software's input file format is a .txt.
Example: 'input_file_name.txt'
The file content for siRNA design:
>Gene name 1
GTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGGT
AGCTGGGATGTTAGGGCTCAGGGAAGAAAAGTCAGAAGACCAGGACCTCCAGGGCCTCAAGGACAAACCC
CTCAAGTTTAAAAAGGTGAAGAAAGATAAGAAAGAAGAGAAAGAGGGCAAGCATGAGCCCTCTCAGCCAT
CAGCCTCTCACTCTGCTGAGCCCGCAGAGGCAGGCAAAGCAGAGACATCAGAAGGGTCAGGCTCCGCCCC
GGCTGTGCCGGAAGCTTCTGCCTCCCCCAAACAGCGGCGCTCCATCATCCGTGACCGGGGACCCATGTAT
GATGACCCTCTCCTGCCTGAAGGCTGGACACGGAAGCTTAAGCAAAGGAAATCTGGCCGCTCTGCTGGGA
AGTATGATGTGTATTTGATCAATCCCCAGGGAAAAGCCTTTCGCTCTAAAGTGGAGTTGATTGCGTACTT
CGAAAAGGTAGGCGACACATCCCTGGACCCTAATGATTTTGACTTCACGGTAACTGGGAGAGGGAGCCCC
TCCCGGCGAGAGCAGAAACTCTCTAAGAAGCCCAAATCTCCCAAAGCTCCAGGAACTGGCAGAGGCCGGG
GACGCCCCAAAGGGAGCGGCACCACGAGACCCAAGGCGGCCACGTCAGAGGGTGTGCAGGTGAAAAGGGT
CCTGGAGAAAAGTCCTGGGAAGCTCCTTGTCAAGATGCCTTTTCAAACTTCGCCAGGGGGCAAGGCTGAG
GGGGGTGGGGCTCTCACATCTCTCCAGGTCATGGTGATCAAACGCCCCGGCAGGAAGCGAAAAGCTGAGG
CCGACCCTCAGGCCATTCCCAAGAAACGGGGCCGAAAGCCGGGGAGTGTGGTGGCAGCCGCTGCCGCCGA
is very important to follow the input structure showed above to avoid any errors
The output file will be a TXT format and will be created in the software's folder.
Example: 'output_file_name_siRNA_Gibbs_SSD_Date_[Hour].txt' for the siRNA designer
'output_file_name_Trivalent_shRNA_Date_[Hour].txt' for the shRNA designer